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Platelet stabilizers, as well as fixative solutions optimized for use with Beckman Coulter’s lysis product portfolio. Cell fixation is accomplished using a cross-linking agent, while permeabilization of white blood cells and lysis of red blood cells is achieved using a detergent. A denaturing agent “unmasks” proteins bound to other proteins or DNA. Beckman Coulter researchers developed the kit for use with whole blood, bone marrow or other fluids containing blood. Samples are fixed in small test tubes or deep-well microplates and the reagent is mixed with the cells. The kit can be used to study a variety of other intracellular and intranuclear antigens.
These innovative, fixation reagent kits for intracellular staining from Beckman Coulter Life Sciences, minimize sample manipulation for a streamlined workflow that delivers high quality results. Developed for use on Beckman Coulter’s Gallios and Navios series flow cytometers and compatible with all Beckman Coulter Life Sciences’ fluorochromes, the kits enable a clear focus on the cytoplasmic and nuclear compartments of the cell.
The IOTest3 Fixative Solution allows whole blood or bone marrow specimen preparation by fixing the cell suspension after erythrolysis during immunophenotyping procedures. At least one wash is required between the lysis and the fixation steps. The IOTest3 Fixative Solution is optimized to work in combination with the IOTest3 Lysing Solution. IOTest Fixative Solution is a formaldehyde-based fixative solution designed for flow cytometry. This reagent is provided as a concentrated solution in PBS.
ThromboFIX Platelet Stabilizer
The ThromboFix Platelet stabilizer extends the window for platelet analysis by ensuring that the platelets cannot be induced to a new functional state, for example, activated. Platelet Stabilizer preserves platelets in their current state at the time of preparation. The ThromboFix Platelet stabilizer may be used in conjunction with platelet monoclonal antibody-dye conjugates for flow cytometric analysis.
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