CytoFLEX nano Flow Cytometer
The CytoFLEX nano Flow Cytometer is the newest member of the CytoFLEX Platform.
Specifically designed for the analysis of nanoparticles, the CytoFLEX nano Flow Cytometer overcomes the limitations of traditional flow cytometry, giving researchers a sensitive, consistent and flexible solution that can help them advance their research.
The CytoFLEX nano Flow Cytometer is a pivotal development in flow cytometry that allows users to analyze extracellular vesicles (EVs) at least as small as 40 nm* with ease while simultaneously offering up to 6 separate fluorescent channels of detection and 5 side scatter channels, to deliver full characterization and open additional avenues for your nanoparticle research.
CytoFLEX nano Flow Cytometer Features
Explore the full picture of your EV experiment with the CytoFLEX nano Flow Cytometer. This purpose-built flow cytometer provides greater sensitivity, consistent instrument performance, and flexibility to study your sample.
High Sensitivity
Flexible EV Analysis
Consistent Performance
Familiar CytoFLEX Platform
| Size | Count | Characterize |
| Particle size determination by flow cytometry requires calibration using an appropriately characterized reference particle paired with a calibration method. Latex polystyrene particles are the tool of choice for this task due to their NIST-traceable nature and reliably consistent manufacturing. Through the calibration method taught to each CytoFLEX nano flow cytometer customer, data will be reliably reportable in absolute units for method comparison and correlation. | The most robust counting methods rely on highly controlled volumetric delivery of samples rather than hand loading samples with the implied variability. The CytoFLEX nano flow cytometer allows users to customize their sample handling so that the right amount of sample is used for the results needed. | The ability to describe nanoscale particles requires best-in-class sensitivity. To make this possible, the CytoFLEX nano flow cytometer has completely redesigned the optics and fluidics system to maximize the detection of even the smallest amounts of fluorescence across 6 detection channels and excited by 4 collinear lasers. |
Performance Characteristics
Explore CytoFLEX nano Models
a. Tested with polystyrene nanoparticles
b. Tested with 144 nm QC Scatterspheres on 3 units under the conditions: record 3 minutes at the sample flow rates of 1µL/min, 2µL/min, 3µL/min, 4µL/min, 5µL/min, 6µL/min respectively, repeat 5 times, then calculate the average total events for each speed, then compare the calculated volumetric counting accuracy with the theoretical total events ( the known concentration 1.3*10 8 x volume).
*polystyrene when triggering on violet side scatter
** In development. Performance characteristics have not been validated.
For Research Use Only. Not for use in diagnostic procedures.
CytoFLEX nano Flow Cytometer FAQs
What is the CytoFLEX nano Flow Cytometer used for?
The CytoFLEX nano Flow Cytometer is used for nanoparticle flow cytometry applications, including the analysis of extracellular vesicles and other small particles.
What is nanoparticle flow cytometry?
Nanoparticle flow cytometry is a technique used to detect and analyze particles smaller than those typically measured by traditional flow cytometers, including extracellular vesicles.
What size particles can the CytoFLEX nano Flow Cytometer detect?
The system enables analysis of particles at least as small as 40 nm, depending on the application.
How does the CytoFLEX nano Flow Cytometer support extracellular vesicle analysis?
The system is designed with sensitivity and detection capabilities suited for analyzing small particles such as extracellular vesicles.
How many fluorescent channels does the CytoFLEX nano Flow Cytometer support?
The system offers up to six separate fluorescent detection channels.
What makes the CytoFLEX nano Flow Cytometer different from traditional flow cytometers?
The CytoFLEX nano is specifically designed for nanoparticle analysis, addressing limitations in detecting very small particles with traditional systems.
How does the CytoFLEX nano Flow Cytometer improve particle characterization?
The system includes five side scatter channels, enabling enhanced characterization of nanoparticles.
What types of research applications is the CytoFLEX nano Flow Cytometer suited for?
The system supports research applications focused on nanoparticles and extracellular vesicles.
Does the CytoFLEX nano Flow Cytometer support multi-parameter analysis?
Yes, the system supports multi-parameter detection through multiple fluorescent and side scatter channels.
