Expand Violet, UV, or Near UV Channels with the CytoFLEX LX WDM Beam Splitter
The CytoFLEX LX Flow Cytometer offers an IR laser option as a standard product; it includes two detectors devoted to emission from this excitation source. Using the WDM beam splitter it will be possible to define these channels to collect emission from either the UV/Near UV or the Violet laser. This will give operators more flexibility for multicolor panels utilizing the commercially available dyes with emission in this part of the spectrum.
The median fluorescence intensity (MFI) of the unsplit signal can be achieved on the analogous split signal by adjusting the gain. To change between configurations, just reposition the fibers and select the appropriate detector configuration in CytExpert Software version 2.4 or higher. For those conducting research in the nanoscale range, the VSSC capability to resolve 80 nm PS particles is retained when the WDM Beam Splitter is engaged in the configuration. Additional bandpass filters are available including a custom bandpass filter holder which can be used for the new channels.